Rodrigo Manjarín, Assistant Professor
Office: Bldg. 10 Rm. 135
Office Phone: 805-756-7474
- D.V.M., Veterinary Medicine, University of Leon (Spain), 2003
- M.S. Animal Science, Michigan State University, 2008
- Ph.D. Animal Science, Michigan State University, 2011
- Ph.D. Veterinary Medicine, University of Leon (Spain), 2013
- Anatomy and Physiology of Farm Animals (ASCI 229)
- Reproductive Physiology (ASCI 351)
- Undergraduate Seminar (ASCI 363)
- Animal Metabolism and Nutrition (ASCI 420)
- Senior Project - Research Experience in Animal Science (ASCI 477)
- Senior Project - Current Topics in Animal Science (ASCI 479)
Our laboratory specializes in translational biomedical research with emphasis on metabolic disease in pediatric populations. The focus within this area is on gut-liver mechanisms that regulate the development of non-alcoholic fatty liver disease and obesity, and the impact of the diet on these mechanisms. We use a comparative approach which includes a novel pig model of the disease, and multi-omic method that combines the analysis metabolomics and microbiome. Also, we make extensive use of next generation sequencing approaches to determine the transcriptome profile.
Dysregulation of bile acid homeostasis in pediatric NAFLD
Changes in Farnesoid-X receptor (FXR)-fibroblast growth factor 19 (FGF19) signaling and cholestasis has been described in nonalcoholic fatty liver disease (NAFLD) patients. However, treatment with FXR agonists have produced contradictory results. In a translational model of pediatric nonalcoholic steatohepatitis (NASH), we have shown that high-fructose high-fat diets are associated with the uncoupling of FXR-FGF19 signaling in the gut and an increase in the severity of liver disease. These findings provide a therapeutic opportunity for dietary interventions to modulate the enterohepatic FXR-FGF19 axis in NAFLD patients.
Colonic hyperplasia in diet-induced NAFLD
Clinical studies have shown an increased risk of colorectal adenoma in patients with NAFLD, and colorectal cancer and NAFLD share the same risk factors, including intake of high fat diets and elevated levels of serum and fecal bile acids. By combining histology, RNA expression, metabolomics and microbiome analysis in colon samples, we have shown changes in colonic bile acid and lipidomic profile to parallel compositional shifts in microbial communities and proliferative changes in intestinal mucosa, providing a link between colonic injury and diet-induced NAFLD.
UNDERGRADUATE AND GRADUATE STUDENT AWARDS
- 1st place 2020 CSU Graduate Student Research Competition, Nicole Zeltser
- 2nd place 2019 ASN Emerging Leader in Nutrition, Gabriella Hernandez
- 2nd place 2019 ASN Young Minority Investigator, Gabriella Hernandez
- 1st place 2019 CSU Graduate Student Research Competition, Victoria Smith
- 2nd place 2019 CAFES (CalPoly) Graduate Research Symposium, Victoria Smith
- 1st place 2018 CAFES (CalPoly) Graduate Research Symposium, Gabriella Hernandez
- 3rd place Undergraduate Oral Student Competition ASAS Midwest 2018, Claudia Macedo
- 2nd place Undergraduate Oral Student Competition ASAS Midwest 2018, Avery Emond
- 3rd place Undergraduate Oral Student Competition ASAS Midwest 2017, Gabriella Hernandez
- 3rd place Undergraduate Poster Student Competition ASAS/JAM 2016, Molly McGuckin
- Maj M, Harbottle B, Thomas PA, Hernandez GV, Smith VA, Edwards MS, Fanter RK, Glanz HS, Immoos C, Burrin DG, Santiago-Rodriguez TM, La Frano MR, Manjarín R. Consumption of high-fructose corn syrup versus sucrose promotes adiposity and increased triglyceridemia but comparable NAFLD severity in juvenile Iberian pigs. J Nutr. 2020; In Press.
- Hernandez GV, Smith VA, Melnyk M, Burd MA, Sprayberry KA, Edwards MS, Peterson DG, Bennet DC, Fanter RK, Columbus DA, Steibel JP, Glanz H, Immoos C, Rice MS, Santiago-Rodriguez TM, Blank J, VanderKelen JJ, Kitts CL, Piccolo BD, La Frano MR, Burrin DG, Maj M, Manjarin R. Dysregulated FXR-FGF19 signaling and choline metabolism are associated with gut dysbiosis and hyperplasia in a novel pig model of pediatric NASH. Am J Physiol Gastrointest Liver Physiol. 2020;318(3):G582-G609.
- Zeltser N, Meyer I, Hernandez GV, Trahan MJ, Fanter RK, Abo-Ismail M, Glanz H, Strand CR, Burrin DG, La Frano MR, Manjarín R, Maj M. Neurodegeneration in juvenile Iberian pigs with diet-induced nonalcoholic fatty liver disease. Am J Physiol Endocrinol Metab. 2020;319(3):E592-E606.
- Manjarin R, Maj MA, La Frano MR, Glanz H. %polynova_2way: A SAS macro for implementation of mixed models for metabolomics data. PLoS One. 2020;15(12):e0244013.
- Manjarín R, Boutry-Regard C, Suryawan A, Canovas A, Piccolo BD, Maj M, Abo-Ismail M, Nguyen HV, Fiorotto ML, Davis TA. Intermittent leucine pulses during continuous feeding alters novel components involved in skeletal muscle growth of neonatal pigs. Amino Acids. 2020;52(9):1319-1335.
- Smith V, Jiang Y, Thymann T, Sangild P, Maj M, Manjarin R, Burrin D. Rapid postnatal upregulation of intestinal farnesoid x receptor-fibroblast growth factor 19 signaling in premature pigs. J Pediatr Gastroenterol Nutr. 2020;70(5):e94-e99.
- Manjarín R, Columbus DA, Solis J, Hernandez-García AD, Suryawan A, Nguyen HV, McGuckin MM, Jimenez RT, Fiorotto ML, Davis TA. Short- and long-term effects of leucine and branched-chain amino acid supplementation of a protein- and energy-reduced diet on muscle protein metabolism in neonatal pigs. Amino Acids. 2018 Jul;50(7):943-959.
- Manjarín R, Suryawan A, Koo SJ, Wilson FA, Nguyen HV, Davis TA, Orellana RA. Insulin modulates energy and substrate sensing and protein catabolism induced by chronic peritonitis in skeletal muscle of neonatal pigs. Pediatr Res. 2016 Nov;80(5):744-752.
Complete List of Published Work: